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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
20/02/2024 |
Actualizado : |
20/02/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
SANGUIÑEDO, P.; FACCIO, R.; ABREO, E.; ALBORÉS, S. |
Afiliación : |
PAULA SANGUINEDO, Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, 11800, Uruguay; RICARDO FACCIO, Centro NanoMat & Grupo Física, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Universidad de la República, Montevideo, 11800, Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA ALBORÉS, Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, 11800, Uruguay. |
Título : |
Biogenic silver and copper nanoparticles: potential antifungal agents in rice and wheat crops. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Chemistry (Switzerland). 2023, 15(4): 2104-2119. https://doi.org/10.3390/chemistry5040143 -- OPEN ACCESS. |
ISSN : |
eISSN 2624-8549 |
DOI : |
10.3390/chemistry5040143 |
Idioma : |
Inglés |
Notas : |
Article history: Submission received: 5 August 2023; Revised: 2 October 2023; Accepted: 4 October 2023; Published: 6 October 2023. -- Academic editor: Simona Concilio -- Supplementary Materials: The following supporting information can be downloaded at - https://www.mdpi.com/article/10.3390/chemistry5040143/s1 -- Funding: This research was funded by Project INNOVAGRO FSA_1_2018_1_152546 (Agencia Nacional de Investigación e Innovación-Instituto Nacional de Investigación Agropecuaria), Posgrado en Biotecnología y Comisión Académica de Posgrado (CAP) (Universidad de la República, Uruguay), Program for the Development of Basic Sciences (PEDECIBA-Química) and Sociedad Uruguaya de Microbiología (SUM). -- Acknowledgments: The authors acknowledge Sebastian Martínez, Sandra Lupo, and Silvia Pereyra, who kindly provided the fungal strains used in this study. -- This article belongs to the Special Issue Recent Advances in Antimicrobial Materials (https://www.mdpi.com/journal/chemistry/special_issues/Antimicrobial_Material ) -- Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- Publisher: Multidisciplinary Digital Publishing Institute (MDPI). |
Contenido : |
ABSTRACT.- Metal nanoparticles are widely studied due to their various applications, such as their potential use in the control of phytopathogens and the promotion of plant growth, with a significant impact on agriculture. Various microbial metabolites are used to reduce and stabilize metals and metal oxides to the nanoscale. In the present work, the biological synthesis of silver and copper oxide nanoparticles using Trichoderma harzianum TA2 is reported. The nanoparticles were purified and characterized with complementary methodologies to obtain information on the size, distribution, morphology, surface charge, and functional groups of the nanoparticles. The in vitro antifungal activity of the nanoparticles against pathogens of rice and wheat, as well as their effect on seed germination, were evaluated. In general, the nanoparticles showed a spherical shape, an average size of 17-26 nm, and low polydispersity. Furthermore, they showed antifungal activity at low concentrations against Sclerotium oryzae (0.140 nM), Rhizoctonia oryzae-sativae (0.140 nM), Fusarium graminearum (0.034 nM), and Pyricularia oryzae (0.034 nM). The germination of seeds treated with nanoparticles was not negatively affected. This is the first report of biogenic silver and copper oxide nanoparticles from a single strain of T.harzianum with antifungal activity against four phytopathogens of interest in Uruguay. Furthermore, the synthesis of the biogenic nanoparticles was faster and more efficient than previous reports using other fungi. In conclusion, this work reveals that biogenic metallic nanoparticles from T. harzianum TA2 can be considered as candidates for the control of phytopathogens affecting important crops.. © 2023 by the authors. MenosABSTRACT.- Metal nanoparticles are widely studied due to their various applications, such as their potential use in the control of phytopathogens and the promotion of plant growth, with a significant impact on agriculture. Various microbial metabolites are used to reduce and stabilize metals and metal oxides to the nanoscale. In the present work, the biological synthesis of silver and copper oxide nanoparticles using Trichoderma harzianum TA2 is reported. The nanoparticles were purified and characterized with complementary methodologies to obtain information on the size, distribution, morphology, surface charge, and functional groups of the nanoparticles. The in vitro antifungal activity of the nanoparticles against pathogens of rice and wheat, as well as their effect on seed germination, were evaluated. In general, the nanoparticles showed a spherical shape, an average size of 17-26 nm, and low polydispersity. Furthermore, they showed antifungal activity at low concentrations against Sclerotium oryzae (0.140 nM), Rhizoctonia oryzae-sativae (0.140 nM), Fusarium graminearum (0.034 nM), and Pyricularia oryzae (0.034 nM). The germination of seeds treated with nanoparticles was not negatively affected. This is the first report of biogenic silver and copper oxide nanoparticles from a single strain of T.harzianum with antifungal activity against four phytopathogens of interest in Uruguay. Furthermore, the synthesis of the biogenic nanoparticles was faster and more efficient than p... Presentar Todo |
Palabras claves : |
Antifungal; Biogenic nanoparticles; Partnership for the goals - Goal 17; Phytopathogens; PLATAFORMA DE BIOINSUMOS - INIA; Sustainable Development Goals (SDGs); Trichoderma. |
Asunto categoría : |
F01 Cultivo |
URL : |
https://www.mdpi.com/2624-8549/5/4/143/pdf
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Marc : |
LEADER 03975naa a2200277 a 4500 001 1064467 005 2024-02-20 008 2023 bl uuuu u00u1 u #d 022 $aeISSN 2624-8549 024 7 $a10.3390/chemistry5040143$2DOI 100 1 $aSANGUIÑEDO, P. 245 $aBiogenic silver and copper nanoparticles$bpotential antifungal agents in rice and wheat crops.$h[electronic resource] 260 $c2023 500 $aArticle history: Submission received: 5 August 2023; Revised: 2 October 2023; Accepted: 4 October 2023; Published: 6 October 2023. -- Academic editor: Simona Concilio -- Supplementary Materials: The following supporting information can be downloaded at - https://www.mdpi.com/article/10.3390/chemistry5040143/s1 -- Funding: This research was funded by Project INNOVAGRO FSA_1_2018_1_152546 (Agencia Nacional de Investigación e Innovación-Instituto Nacional de Investigación Agropecuaria), Posgrado en Biotecnología y Comisión Académica de Posgrado (CAP) (Universidad de la República, Uruguay), Program for the Development of Basic Sciences (PEDECIBA-Química) and Sociedad Uruguaya de Microbiología (SUM). -- Acknowledgments: The authors acknowledge Sebastian Martínez, Sandra Lupo, and Silvia Pereyra, who kindly provided the fungal strains used in this study. -- This article belongs to the Special Issue Recent Advances in Antimicrobial Materials (https://www.mdpi.com/journal/chemistry/special_issues/Antimicrobial_Material ) -- Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- Publisher: Multidisciplinary Digital Publishing Institute (MDPI). 520 $aABSTRACT.- Metal nanoparticles are widely studied due to their various applications, such as their potential use in the control of phytopathogens and the promotion of plant growth, with a significant impact on agriculture. Various microbial metabolites are used to reduce and stabilize metals and metal oxides to the nanoscale. In the present work, the biological synthesis of silver and copper oxide nanoparticles using Trichoderma harzianum TA2 is reported. The nanoparticles were purified and characterized with complementary methodologies to obtain information on the size, distribution, morphology, surface charge, and functional groups of the nanoparticles. The in vitro antifungal activity of the nanoparticles against pathogens of rice and wheat, as well as their effect on seed germination, were evaluated. In general, the nanoparticles showed a spherical shape, an average size of 17-26 nm, and low polydispersity. Furthermore, they showed antifungal activity at low concentrations against Sclerotium oryzae (0.140 nM), Rhizoctonia oryzae-sativae (0.140 nM), Fusarium graminearum (0.034 nM), and Pyricularia oryzae (0.034 nM). The germination of seeds treated with nanoparticles was not negatively affected. This is the first report of biogenic silver and copper oxide nanoparticles from a single strain of T.harzianum with antifungal activity against four phytopathogens of interest in Uruguay. Furthermore, the synthesis of the biogenic nanoparticles was faster and more efficient than previous reports using other fungi. In conclusion, this work reveals that biogenic metallic nanoparticles from T. harzianum TA2 can be considered as candidates for the control of phytopathogens affecting important crops.. © 2023 by the authors. 653 $aAntifungal 653 $aBiogenic nanoparticles 653 $aPartnership for the goals - Goal 17 653 $aPhytopathogens 653 $aPLATAFORMA DE BIOINSUMOS - INIA 653 $aSustainable Development Goals (SDGs) 653 $aTrichoderma 700 1 $aFACCIO, R. 700 1 $aABREO, E. 700 1 $aALBORÉS, S. 773 $tChemistry (Switzerland). 2023, 15(4): 2104-2119. https://doi.org/10.3390/chemistry5040143 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
17/05/2022 |
Actualizado : |
17/05/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PALLADINO, C.; MUELA, A.; TABORDA, B.; PUIGVERT, F.; PÉREZ-PARADA, A.; COLAZZO, M.; PÉREZ, C. A.; PEREYRA, S.; PAREJA, L. |
Afiliación : |
CINTIA PALLADINO, PDU Abordaje Holístico Al Impacto de Los Agroquímicos, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, 60000, Uruguay; AGUSTINA MUELA, Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, 60000, Uruguay; BELÉN TABORDA, Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, 60000, Uruguay; FLORENCIA PUIGVERT, Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, 60000, Uruguay; ANDRÉS PÉREZ-PARADA, Departamento de Desarrollo Tecnológico - DDT, Centro Universitario Regional Del Este, Universidad de la República, Ruta 9 y Ruta 15, 27000, Rocha, Uruguay; MARCOS COLAZZO, Licenciatura en Análisis Alimentario, Inst. Tecnologico Regional Suroeste, Universidad Tecnologica, Calle 17, Saladero Santa Maria, 60000, Paysandu, Uruguay; CARLOS A. PÉREZ, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, EEMAC, Ruta 3 km 363, Paysandu, 60000, Uruguay; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA PAREJA, Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, 60000, Uruguay. |
Título : |
Application of a straightforward Mmthodology by LC-QqQ-MS/MS and GC-MS to ensure food safety of barley and wheat grain. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
ACS Agricultural Science and Technology, 2022, Volume 2, Issue 2, Pages 371-380. doi: https://doi.org/10.1021/acsagscitech.1c00263 |
ISSN : |
2692-1952 |
DOI : |
10.1021/acsagscitech.1c00263 |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 November 2021; Accepted 24 February 2022; Revised 4 February 2022; Published online 16 March 2022; Published in issue 18 April 2022.
Corresponding author: Pareja, L.; Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, Uruguay; email:lpareja@fq.edu.uy -- |
Contenido : |
ABSTRACT. A multiclass methodology to determine deoxynivalenol, zearalenone, and 13 widely used fungicides to control the main diseases in barley and wheat crops was optimized and validated. Three QuEChERS methods were compared in terms of recovery and repeatability. The recoveries of the validated method were in the 67-128% range for the selected compounds and relative standard deviations were below 20% according to Document SANTE No. 12682/2019. The limits of quantification (LOQs) were between 10 and 100 μg kg-1. The method was applied for the analysis of barley and wheat grains obtained under production conditions. These samples contained mycotoxins and fungicide residues at concentrations below the LOQs, except for carbendazim in wheat, which exceeded the Codex maximum residue levels. The results of this study provide a fast and simple analytical tool to evaluate the primary production of these grains and to identify possible risks to human health.
© 2022 American Chemical Society. |
Palabras claves : |
Cereals; Food safety; Fungicide residues; Hyphenated chromatographic techniques; Mycotoxins. |
Thesagro : |
CEREALES. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02367naa a2200325 a 4500 001 1063147 005 2022-05-17 008 2022 bl uuuu u00u1 u #d 022 $a2692-1952 024 7 $a10.1021/acsagscitech.1c00263$2DOI 100 1 $aPALLADINO, C. 245 $aApplication of a straightforward Mmthodology by LC-QqQ-MS/MS and GC-MS to ensure food safety of barley and wheat grain.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 29 November 2021; Accepted 24 February 2022; Revised 4 February 2022; Published online 16 March 2022; Published in issue 18 April 2022. Corresponding author: Pareja, L.; Departamento de Química Del Litoral, Cenur Litoral Norte, Universidad de la República, Ruta 3 km 363, Paysandu, Uruguay; email:lpareja@fq.edu.uy -- 520 $aABSTRACT. A multiclass methodology to determine deoxynivalenol, zearalenone, and 13 widely used fungicides to control the main diseases in barley and wheat crops was optimized and validated. Three QuEChERS methods were compared in terms of recovery and repeatability. The recoveries of the validated method were in the 67-128% range for the selected compounds and relative standard deviations were below 20% according to Document SANTE No. 12682/2019. The limits of quantification (LOQs) were between 10 and 100 μg kg-1. The method was applied for the analysis of barley and wheat grains obtained under production conditions. These samples contained mycotoxins and fungicide residues at concentrations below the LOQs, except for carbendazim in wheat, which exceeded the Codex maximum residue levels. The results of this study provide a fast and simple analytical tool to evaluate the primary production of these grains and to identify possible risks to human health. © 2022 American Chemical Society. 650 $aCEREALES 653 $aCereals 653 $aFood safety 653 $aFungicide residues 653 $aHyphenated chromatographic techniques 653 $aMycotoxins 700 1 $aMUELA, A. 700 1 $aTABORDA, B. 700 1 $aPUIGVERT, F. 700 1 $aPÉREZ-PARADA, A. 700 1 $aCOLAZZO, M. 700 1 $aPÉREZ, C. A. 700 1 $aPEREYRA, S. 700 1 $aPAREJA, L. 773 $tACS Agricultural Science and Technology, 2022, Volume 2, Issue 2, Pages 371-380. doi: https://doi.org/10.1021/acsagscitech.1c00263
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